The company has various types of host cells, with clear cell sources and no pollution. It provides a variety of options to express foreign proteins and process optimization
Plasmid construction (outsourcing): integrate foreign genes into the plasmid skeleton for the expression of target genes in cells. The company can provide multiple plasmid skeleton selection, different pressure screening systems, and optimize plasmid elements and foreign target genes. The plasmid expression performance was tested and compared by transient or large pool.
It can be divided into transient transfection and stable transfection. The main methods are liposome transfection and electrotransfection. Objective to introduce foreign DNA fragments into cells under certain conditions to obtain a new phenotype and release the target protein to the outside of the cell. A large number of cell transfection experiments can be carried out in the laboratory, and the target protein can be quickly obtained by transient transfection or the stable protein secreting monoclonal cell line can be obtained by pressurization.
The plasmid was introduced into the host cell by transfection and integrated into the chromosome. The cell line was screened with the corresponding resistance markers in the plasmid DNA. A series of screening criteria and mechanisms were used to obtain a high-yield cell line and expand it. Finally, monoclonal cell lines were prepared to obtain highly secreted foreign protein cell lines with stable properties. At present, the stable cell line of platform technology can reach >4g/L.
The positive cell group obtained after transfection can be separated into single cells by limited dilution method or modern machine and grow, which can obtain high-yield positive cell lines and eliminate non expressing or low expressing cells, and the single cell group has high stability. During the experiment, a monoclonal imager was used for the whole process of tracking, photographing and imaging, and a monoclonal report was issued to ensure the accuracy of the experiment.
The stability of high-yield monoclonal cell lines was tested, and the cells were subcultured for more than 40 generations. The growth and metabolism of cells were compared, and the amount of protein secretion and protein quality were considered. Now it can be stably passed on for more than 40 generations.
The company has various types of host cells, with clear cell sources and no pollution. It provides a variety of options to express foreign proteins and process optimization
Plasmid construction (outsourcing): integrate foreign genes into the plasmid skeleton for the expression of target genes in cells. The company can provide multiple plasmid skeleton selection, different pressure screening systems, and optimize plasmid elements and foreign target genes. The plasmid expression performance was tested and compared by transient or large pool.
It can be divided into transient transfection and stable transfection. The main methods are liposome transfection and electrotransfection. Objective to introduce foreign DNA fragments into cells under certain conditions to obtain a new phenotype and release the target protein to the outside of the cell. A large number of cell transfection experiments can be carried out in the laboratory, and the target protein can be quickly obtained by transient transfection or the stable protein secreting monoclonal cell line can be obtained by pressurization.
The plasmid was introduced into the host cell by transfection and integrated into the chromosome. The cell line was screened with the corresponding resistance markers in the plasmid DNA. A series of screening criteria and mechanisms were used to obtain a high-yield cell line and expand it. Finally, monoclonal cell lines were prepared to obtain highly secreted foreign protein cell lines with stable properties. At present, the stable cell line of platform technology can reach >4g/L.
The positive cell group obtained after transfection can be separated into single cells by limited dilution method or modern machine and grow, which can obtain high-yield positive cell lines and eliminate non expressing or low expressing cells, and the single cell group has high stability. During the experiment, a monoclonal imager was used for the whole process of tracking, photographing and imaging, and a monoclonal report was issued to ensure the accuracy of the experiment.
The stability of high-yield monoclonal cell lines was tested, and the cells were subcultured for more than 40 generations. The growth and metabolism of cells were compared, and the amount of protein secretion and protein quality were considered. Now it can be stably passed on for more than 40 generations.
